Table 1.
Parameter | ΔG(kcal/mol) | ± |
---|---|---|
OR1* | −12.5 | 0.5 |
OR2* | −10.5 | 0.5 |
OR3* | −9.5 | 0.5 |
OR3-r1†‡ | −6.6 | 0.5 |
OR12coop* | −2.7 | 0.5 |
OR23coop* | −2.9 | 0.5 |
OL1§ | −13.0 | 0.5 |
OL2§ | −11.2 | 0.5 |
OL3§ | −12.0 | 0.5 |
OL3–4‡ | see ΔGns | |
OL12coop§ | −2.7 | 0.5 |
OL23coop§ | −2.0 | 0.5 |
Δ Goct†‡ | −0.5 | 0.5 |
Δ Gtet†‡ | −3.0 | 0.5 |
Δ Gns‡¶ | −4.1 | 0.9 |
We varied all parameters in increments of 0.1 kcal/mol over the range given in the table, even though the reported uncertainty estimates for several of the experimental values were smaller than 0.5 kcal/mol.
*From ref. 13.
†From estimates calculated in ref. 7.
‡Values that have not been directly measured.
§From data presented in figure 1B of ref. 12, recalculated with a dimerization free energy ΔGdim = −11.0 kcal/mol (36) and setting OL12coop = OR12coop = −2.7 kcal/mol. The values for OL cooperativity were not unambiguously determined by Senear et al. (12), but the chosen values reflect our assumptions.
¶From estimates calculated in ref. 35.