Fig. 2.

Reproducibility and sensitivity of GIM. (A) Comparison of two independent screens with edc3Δ as a query mutation and ymr326cΔ as a reference. Arrows point to the values obtained for mutants that were subsequently tested on plates. (B) Plate assays for double deletions with edc3Δ. Dilution series of sporulated cultures of double mutants obtained by combining either the query mutation edc3Δ::prMFα2Nat^R (arrowhead) or the control mutation ymr326cΔ::prMFα2Nat^R (asterisk) and the indicated deletions (“Control” represents the deletion of YEL068C, another reference). Cultures were grown at 23°C on rich medium containing geneticin and nourseothricin. The deletion of the dubious ORF YPR130C was called “scd6Δ*” because it overlaps the scd6Δ deletion and thus constitutes an independent mutation of SCD6. (C) As in A except that lsm12Δ was used as the query mutation. (D) Matrix of genetic interactions between genes whose deletions showed a synthetic growth defect with edc3Δ. The values are means of at least two independent screens. The plus sign after edc3Δ and scd6Δ indicates that the shown values were averaged from edc3Δ and yel014cΔ and from scd6Δ and ypr130cΔ, respectively.