Skip to main content
. 2008 Apr 7;105(15):5856–5861. doi: 10.1073/pnas.0801677105

Fig. 1.

Fig. 1.

In vitro differentiation of induced pluripotent stem cells. (a) The undifferentiated Oct4-selected iPS cell line O9. (b) FGF2-responsive neural precursor cells. (c) Differentiated neural morphologies 7 days after growth factor withdrawal. (d) A fraction of β-III-tubulin positive neurons (red) are double labeled with antibodies against TH (green, yellow in merged image), 7 days after growth factor withdrawal. (e and f) At this stage, also, many GFAP-positive astrocytes (red) (e) and rare 04-positive oligodendrocytes (f) are found. (g) The fraction of TH-positive cells over β-III-tubulin positive cells increases along neuronal maturation (mean and standard deviation, three independent experiments). (h) The vast majority of TH-immunoreactive cells coexpress En1, Pitx3, and Nurr1 (mean, standard deviation). (i) Coexpression of En1 (green) and TH (red). (j) VMAT2 and TH [purple in the merged image indicates colocalization of TH (red) and VMAT2 (blue)]. (k and l) Most TH-positive cells (red) are also positive for Pitx3 (green) (k), and Nurr1 (green) (l) after 7 days of neuronal differentiation. [Scale bar in a: 200 μm (a and b), 100 μm (c, d, i, and j), 50 μm (e and k), and 20 μm (f and l).]