Figure 5.

tTS-Enhanced Heterochromatin Assembly and CENP-A Chromatin Disassembly on the Alphoid^tetO HAC

AB2.2.18.21 cells expressing tetR or tTS were cultured in doxycycline-free medium for 7 or 14 d and analyzed by ChIP with antibodies against CENP-A and modified histone H3. CENP-A assembly on alphoid^tetO dimer was gradually decreased following induction of tTS binding for 7–14 d. Levels of H3K4me2 and H3K4me3 on the alphoid^tetO HAC decreased rapidly upon tTS binding. In contrast, H3K9me3 levels on the alphoid^tetO HAC increased transiently following tTS binding, but then declined again (possibly due to loss of the HAC). Error bars indicate SD (n = 2–3).