Fig. 1.

Bolus and glucose oxidase generated H₂O₂ mediate CREB phosphorylation in an oxidant-dependent manner. (a) C10 cells, pre-incubated with 1,000 U/ml catalase (CAT) for 1 h, were treated with 10 μM forskolin or 100, 200, or 300 μM H₂O₂ for 10 min, and then analyzed by Western blot analysis for phospho-CREB (p-CREB). (b) C10 cells, pre-incubated with 10 mM NAC for 16 h or 1,000 U/ml catalase (CAT) for 1 h, were treated with 10 μM forskolin or 5, 15, or 30 mU/ml glucose oxidase (GO) for 4 h, and then analyzed by Western blot analysis for phospho-CREB (p-CREB). An antibody recognizing total CREB was used as a control for protein loading in A and B. Data are representative of 3 separate experiments