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. 2002 Apr;119(4):341–354. doi: 10.1085/jgp.20028565

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Voltage dependence of Pf in a detached rod outer segment isolated from tiger salamander retina. Data shown were measured in the same cell in successive trials at either −65 mV or −25 mV membrane voltage. Intracellular 8-Br-cGMP generated through flash photolysis of a caged compound (flash delivery indicated by arrow) activated inward membrane currents (top) and a decrease in fluorescence (illustrated with symbols as an upward change in the middle panels. In the middle panels, the continuous line superimposed on the fluorescence change is the time integral of the current adjusted in amplitude to best fit the fluorescence signal. The bottom panel illustrates the mean (± SD) value of Pf measured at various membrane voltages.