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Survival of PNH cells lines in serum-free medium. (A) LD− and LD− (PIG-A+). (B) JY-5 and JY-5 (PIG-A+). Cell viability was determined by trypan blue exclusion after incubation in serum-free medium for 48 hr. The data are the mean of ± SEM of three separate experiments. (C) Flow cytometric analysis for apoptosis of LD− and LD− (PIG-A+) cells after 48 hr in serum-free medium.
