TABLE I.

Effects of Mutations on the G-V Relationships

	G-V
	V 0.5	q
	mV	e0
WT	−50.0 ± 1.2	5.2 ± 0.3 (n = 4)
N482C	−44.6 ± 2.4	5.2 ± 0.4 (n = 4)
Y483C	−54.4 ± 2.8	4.6 ± 0.2 (n = 5)
F484C	−36.6 ± 5.3	2.3 ± 0.7 (n = 4)
Y485C	−48.8 ± 1.1	4.1 ± 0.5 (n = 4)
H486C	−55.9 ± 1.2	4.3 ± 0.6 (n = 5)
R487C	−54.0 ± 1.0	4.7 ± 0.4 (n = 3)
E488C	−50.0 ± 3.1	4.8 ± 0.6 (n = 4)
T489C	−50.7 ± 2.2	5.8 ± 0.3 (n = 4)

Estimated parameters from fits of G-V curves to single Boltzmann functions for K⁺ currents in outside-out patches (Fig. 2).