Fig. 5.

Interaction of dex, cAMP, and insulin in controlling mRNA expression for peroxisome proliferator-activated receptor α (PPARα) and peroxisome proliferator-activated receptor-coactivator-1α (PGC-1α) in mouse hepatocytes. Mouse hepatocytes were incubated for the times shown with 100 nM dex (Dex), 100 μM CPTcAMP (cAMP), and 100 nM insulin (Ins) alone or in combination as indicated. Relative mRNA concentrations for PGC-1α (A, B) and PPARα (C, D) were measured by real-time RT-PCR and expressed relative to that for cyclophilin A. Results are expressed as means ± SEM for three independent experiments. The significance of the differences (P < 0.05) is indicated as follows: * different from the untreated control value; ^§ dex alone different from dex + CPTcAMP treatment.