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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Biol Reprod. 2007 Dec 5;78(3):455–461. doi: 10.1095/biolreprod.107.064964

FIG. 7.

FIG. 7

Gel-shift assay demonstrating specific interaction of Ldhc promoter oligos containing GC box (Ldhc-SP) or CRE sequences (Ldhc-CRE) with testis nuclear protein extract (TN). Lanes 1 to 7, oligo Ldhc-SP used as probe; lane 1: Ldhc-SP probe; lane 2: TN + probe; lanes 3–5: TN + probe + 50-fold non-radioactive Ldhc-SP, Ldhc-SPmu, and SP1 consensus, respectively; lanes 6–7: TN + probe + antibody. Lanes 8–14, oligo Ldhc-CRE used as probe; lane 8: Ldhc-CRE; lane 9: TN + probe; lanes 10–12: TN + probe + 50-fold non-radioactive Ldhc-CRE, Ldhc-CREmu1, and CRE consensus, respectively; lanes 13–14: TN + probe + antibody. NS, Nonspecific.