TABLE 1.
Analysis of transgenic founders and offspring.
| β-Gal reporter expressionb |
|||
|---|---|---|---|
| Founder linea | Founder | Pups | Transgene copy no. |
| PALmu | |||
| 323 (M) | 4 Positive | 5 | |
| 321 (M) | Positive | ||
| 327 (M) | Positive | ||
| 330 (F) | 1 Positive | 24 | |
| SPmu | |||
| 230 (M) | Positive | ||
| 260 (M) | 1 Positive | 4 | |
| 272 (M) | 3 Positive | 5 | |
a
M, Male; F, Female.
b
Positive β-gal expression determined by visible X-gal staining in testis tubules; significant level of β-gal activity in testis compared to background level in somatic tissues; and by IHC staining of testis paraffin section by β-gal antibody.