Figure four.
Determination of the time of nuclear envelope breakdown following LH application to isolated antral follicles, or injection of a Gs inhibitory antibody into follicle-enclosed oocytes. A. Percentage of oocytes that have undergone nuclear envelope breakdown at various times after treatment of antral follicles with 1 μg/ml LH. Numbers in parentheses indicate the number of oocytes scored for each point; results were obtained from 13 mice. B. Transmitted light image of an antral follicle-enclosed oocyte on a Millicell membrane, as used to determine the time to nuclear envelope breakdown following injection of the Gs-inhibitory antibody. C. Nuclear envelope breakdown as a function of time after injection of the Gs-inhibitory antibody. Each symbol represents an individual oocyte.