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. 1999 Oct 26;96(22):12828–12832. doi: 10.1073/pnas.96.22.12828

Figure 4.

Figure 4

Immunoblot analysis of CS1 pili produced in wild-type and cooD mutant strains. CS1 pili recovered from the cell surfaces of strains LMG194/pEU1290/pHSG576 (cooBAC) (lanes 1–3), LMG194/pEU1290/pEU493 (cooBAC cooD) (lanes 4–6), LMG194/pEU1290/pEU1227 (cooBAC cooD1) (lanes 7–9), and LMG194/pEU1290 (cooBAC cooD7) (lanes 10–12) were detected by immunoblot analysis with anti-CooA antiserum. Lanes 2, 5, 8, and 11 contain 2-fold-diluted samples. Lanes 3, 6, 9, and 12 contain 4-fold-diluted samples.