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Hemagglutination (HA) and assembly of CS1 pili in LMG194/pEU1290 (cooBAC) complemented with wild-type and mutant alleles of cooD
| Allele | Mutation | Plasmid | Pili* | HA |
|---|---|---|---|---|
| — | — | pHSG576 | − | − |
| cooD | — | pEU493 | + | + |
| cooD1 | R181A | pEU1227 | + | − |
| cooD2 | R219A | pEU1222 | + | + |
| cooD4 | R314A | pEU1224 | + | + |
| cooD5 | R105A | pEU1225 | + | + |
| cooD6 | R110A | pEU1226 | + | + |
| cooD7 | R20A | pEU1228 | + | +/− |
| cooD8 | R37AR40A | pEU1229 | +/− | − |
Indicated by slide agglutination test.
