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. 2008 Apr 25;4(4):e1000060. doi: 10.1371/journal.pgen.1000060

Figure 3. Measurements of NHEJ ability of essential DNA polymerases.

Figure 3

The NHEJ assay was performed for pol1, pol3, pol2-18 and pol2-18 pol4 at permissive (23°C) and semi-permissive (30°C) temperatures. (A) Frequencies of imprecise end joining. The survival frequency was evaluated by the ratio of YPGal/YPD. (B) Frequency of chromosomal rearrangement was evaluated by the ratio of FOAR/YPD. Each experiment was collected from at least five independent clones. Dark gray bars indicate 23°C incubation and light gray bars indicate 30°C incubation. *: P<0.05; **: P<0.01. (C) Survivors of pol2-18 or pol2-4 mutants from YPGal plates at 30°C were examined. Bases underlined indicate insertion or mutation. The numbers of inserted (+) and deleted (−) bases were denoted in brackets. N indicates the events of each repair pattern.