Table 2. Repair patterns after a DSB at the URA3::ACT1 intron::HO cut site locus of essential DNA polymerase mutants.
Strain | Tm(°C) | Imprecise end joininga(%) | Chromosomal rearrangementsb(%) | ||||||||
+CA | −ACA | +base | −base | Others | Nc | insertions | deletions | mutation | Nc | ||
WT | 23 | 15 | 25 | 35 | 65 | 0 | 20 | 75 | 25 | 0 | 20 |
30 | 40 | 25 | 40 | 50 | 10 | 20 | 50 | 50 | 0 | 20 | |
pol1 | 23 | 35 | 35 | 50 | 50 | 0 | 20 | 55 | 45 | 0 | 40 |
30 | 40 | 35 | 60 | 40 | 0 | 20 | 60 | 40 | 0 | 40 | |
pol3 | 23 | 25 | 40 | 45 | 55 | 0 | 20 | 80 | 20 | 0 | 40 |
30 | 30 | 35 | 40 | 60 | 0 | 20 | 80 | 20 | 0 | 40 | |
pol2-18 | 23 | 42 | 28 | 64 | 36 | 0 | 50 | 75 | 7 | 18 | 40 |
30 | 71 | 4.7 | 83 | 17 | 0 | 42 | 88 | 7 | 5 | 40 | |
pol2-18 | 23 | 0 | 80 | 0 | 100 | 0 | 50 | 60 | 7 | 33 | 40 |
pol4 | 30 | 0 | 74 | 0 | 100 | 0 | 50 | 53 | 17 | 30 | 40 |
Analysis of survivors grown on YPGal plates.
Analysis of survivors grown on 5-FOA plates.
Total events examined.