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. 2008 Apr 25;4(4):e1000060. doi: 10.1371/journal.pgen.1000060

Table 2. Repair patterns after a DSB at the URA3::ACT1 intron::HO cut site locus of essential DNA polymerase mutants.

Strain Tm(°C) Imprecise end joininga(%) Chromosomal rearrangementsb(%)
+CA −ACA +base −base Others Nc insertions deletions mutation Nc
WT 23 15 25 35 65 0 20 75 25 0 20
30 40 25 40 50 10 20 50 50 0 20
pol1 23 35 35 50 50 0 20 55 45 0 40
30 40 35 60 40 0 20 60 40 0 40
pol3 23 25 40 45 55 0 20 80 20 0 40
30 30 35 40 60 0 20 80 20 0 40
pol2-18 23 42 28 64 36 0 50 75 7 18 40
30 71 4.7 83 17 0 42 88 7 5 40
pol2-18 23 0 80 0 100 0 50 60 7 33 40
pol4 30 0 74 0 100 0 50 53 17 30 40
a

Analysis of survivors grown on YPGal plates.

b

Analysis of survivors grown on 5-FOA plates.

c

Total events examined.