Table 3. Yeast strains used in this study.
Strain | Genotype | Source |
AGY628 | ho,hml::ADE1,mata::hisG, hmr::ADE, ade1, lys5, trp1::hisG, ade3::GAL-HO, ura3-52, LEU2, intronless ACT1 | [44] |
AGY673 | AGY628 URA3::ai::HO cut site | [44] |
YHA322 | MATα pol2-3::LEU2 leu2-3,112 ura3-52 trp1-289 his4 [YCppol2-18] | [46] |
STY891 | AGY673 pol4::TRP1 | This study |
STY890 | AGY673 rev3::TRP1 | This study |
STY969 | AGY673 pol1 (cdc17-1) | This study |
STY970 | AGY673 pol3 (cdc2-2) | This study |
STY966a | AGY673 pol2::LEU2 [YCppol2-18] | This study |
STY1358 | AGY673 pol2-4 | This study |
STY1359 | AGY673 pol3-01 | This study |
STY1356b | AGY673 pol2::LEU2 [YCppol2-18] pol4::TRP1 | This study |
STY1360c | AGY673 pol2-4 pol4::TRP1 | This study |
STY1544 | AGY673 dnl4::KanMX4 | This study |
STY1546 | AGY673 pol2-4 dnl4::KanMX4 | This study |
STY1549 | AGY673 pol3 (cdc2-2) ura3Δ | This study |
STY1550 | AGY673 pol1 (cdc17-1) ura3Δ | This study |
STY1551 | AGY673 pol2::LEU2 [YCppol2-18] ura3Δ | This study |
STY1552 | AGY673 pol3 (cdc2-2) URA3::ai | This study |
STY1553 | AGY673 pol1 (cdc17-1) URA3::ai | This study |
STY1554 | AGY673 pol2::LEU2 [YCppol2-18] URA3::ai | This study |
From three backcrosses of YHA322 with AGY673.
pol4 of STY966.
pol4 of STY1358.