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. 2008 Feb;172(2):345–357. doi: 10.2353/ajpath.2008.070696

Figure 1.

Figure 1

Colocalization of human CR-1 and Cav-1 in low-density sucrose fractions in 293 and COS7 cells. Western blot analysis on 5% to 40% sucrose gradient fractions isolated from 293 cells transiently transfected with expression plasmids encoding human CR-1 and Alk4-Flag (A) or from COS7 cells transiently transfected with a human CR-1 expression vector (B). Western blot analysis was performed using anti-Flag monoclonal antibody to detect Alk4-Flag fusion protein, anti-CR-1 mouse monoclonal antibody, anti-Cav-1 mouse monoclonal antibody, and anti-β-actin mouse monoclonal antibody. The lipid raft marker GM1 was detected with horseradish peroxidase-conjugated CT-B by dot blot. Low-density sucrose fractions: 4, 5, and 6.