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. 2008 Feb;172(2):345–357. doi: 10.2353/ajpath.2008.070696

Figure 7.

Figure 7

Loss of Cav-1 enhances migration and invasion and induces hyperactivation of MAPK and c-src signaling molecules in primary mammary epithelial cells derived from Cav-1 null/MMTV-CR-1 double transgenic mice. A: Western blot analysis for Cav-1, CR-1, and β-actin in cell lysates derived from primary mammary epithelial cells isolated from FVB/N, Cav-1+/−/CR-1, and Cav-1−/−/CR-1 mice. Migration (B) and invasion (C) assay of FVB/N, Cav-1+/−/CR-1, and Cav-1−/−/CR-1 primary mammary epithelial cells. Cav-1−/−/CR-1 primary mammary epithelial cells were also treated with cavtratin (10 μmol/L) or with a control peptide (10 μmol/L). *P < 0.05 compared to FVB/N cells; **P < 0.05 compared to Cav-1+/−/CR-1 cells; ***P < 0.05 compared to control 10 μmol/L treated Cav-1−/−/CR-1 cells. D: Protein lysates from FVB/N, Cav-1+/−/CR-1, and Cav-1−/−/CR-1 primary mammary epithelial cells were subjected to immunoblotting with antibodies directed against phospho- and total-MAPK and phospho- and total-c-src.