Figure 5.

CCR1^−/− T cells have reduced cytokine production and proliferation in response to restimulation with allergen or virus. A–D: T cells isolated from the draining lymph node of exacerbated animals were restimulated with either RSV- or CRA-pulsed BMDCs for 48 hours. Shown is one representative of three experiments. ^#P = 0.0022, ^##P < 0.0001, *P < 0.0001, **P = 0.0038, ***P = 0.0022. E and F: Proliferation of CD4⁺ and CD8⁺ T cells isolated from the lymph nodes of CCR1^−/− or wild-type mice. T cells (2.0 × 10⁵) were co-cultured with 4.0 × 10⁴ pulsed BMDCs for 5 days and pulsed with ³H thymidine 24 hours before analysis. *P < 0.001 by one-way analysis of variance when compared to WT CD8⁺ T cells cultured with CRA-pulsed BMDCs. n = three mice/group/experiment, data are pooled from four experiments. G: Transfer of wild-type CD4⁺ or CD8⁺ T cells into CCR1^−/− mice restores AHR. ^#P < 0.05 compared to CCR1^−/− *P < 0.01 compared to CCR1^−/−. n = four mice/group/experiment, data are pooled from three experiments.