Figure 6.

CCR1 is associated with cytokine production from CD4⁺ T cells. Lymph node cells (2.0 × 10⁶) were taken from exacerbated wild-type mice 8 hours after allergen challenge and stimulated with PMA and ionomycin for 6 hours in the presence of brefeldin A. Intracellular staining for IL-4, IL-13, and IFN-γ was performed to assess cytokine production. A, C, E: Percentage of total CD4⁺ T cells producing IL-4, IL-13, or IFN-γ. B, D, F: Percentage of CCR1⁺ CD4⁺ T cells producing these same cytokines. G: Quantification of the percentage of total and CCR1⁺ CD4⁺ T cells producing cytokines. *P = 0.0015 **P = 0.0025. F: Percentage of total or CCR1⁺ CD8⁺ T cells producing cytokines. *P = 0.0001. ND = not detected n = three mice/group/experiment, data are pooled from two experiments.