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. 2008 Feb;172(2):367–777. doi: 10.2353/ajpath.2008.070532

Figure 4.

Figure 4

Short-term heat exposure inhibited GM-CSF- and IL-8-induced neutrophil migration via ERK and PI3-K/Akt. To test which signaling pathways control neutrophil migration at 37°C conditions, cells were pretreated with dimethyl sulfoxide control (CTRL = buffer, black columns), 10 μmol/L SB202190 (SB), 10 μmol/L SP600125 (SP), 25 μmol/L PD98059 (PD), or 10 μmol/L LY294002 (LY) for 30 minutes (all inhibitors in white columns). Thereafter migration toward 5 ng/ml GM-CSF (top, n = 5) or 100 nmol/L IL-8 (bottom, n = 5) was measured. The number of experiments reflects independent experiments performed in duplicates. Data are mean ± SEM. *P < 0.05, **P < 0.01.