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. 2008 Feb;172(2):395–405. doi: 10.2353/ajpath.2008.070870

Figure 5.

Figure 5

Exogenous galectin-3 enhances phagocytosis of S. pneumoniae by both WT and galectin-3−/− neutrophils. A: Representative dot blots from WT and galectin-3−/− mouse bone marrow neutrophil preparations gated on all populations (R1). Representative histograms demonstrate ∼80% GR-1 positivity within the R1 gate. B: WT (white bars) and galectin-3−/− (black bars) bone marrow neutrophils were prepared as described in the Materials and Methods. Neutrophils were incubated with 30 μg/ml of recombinant mouse galectin-3 or PBS (control) for 1 hour at 37°C then incubated with a 10:1 ratio of opsonized FITC-S. pneumoniae for 1 hour at 37°C. Samples were gated on GR-1-positive cells and analyzed using a BD FACSCalibur (n = 3) (**P < 0.01 compared to untreated).