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Phosphopeptide mapping of OCC-CT. A: Amino acid sequence of purified OCC-CT. The underlined portions were determined by LC/MS/MS. No significant phosphorylation sites were detected. B: Phosphorylation of synthetic peptides corresponding to mouse OCC 378-387 with T382 (peptide 1), 500-510 with S507A mutation (peptide 2), and 500-510 with S504A mutation (peptide 3) by purified GST-RhoK. The autoradiogram shows phosphorylation of peptides 1 and 3.
