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. 2008 Feb;172(2):521–533. doi: 10.2353/ajpath.2008.070076

Figure 5.

Figure 5

Phosphopeptide mapping of CLD5-CT peptide. A: Amino acid sequence of CLD5-CT peptide. The underlined portion was determined by LC/MS/MS. The phosphorylation site (T207) is in bold. MS/MS fragmentation of trypsin-digested peptide fragment (mouse CLD5 205-218) detected T207 phosphorylation (Ions score, 47; expectation, 1.1e-0.05). B: Phosphorylation of synthetic peptides corresponding to mouse clauding-5 199-208 (peptide 4), and 199-218 (peptide 5) by purified GST-RhoK. The autoradiogram shows phosphorylation of peptide 5.