Skip to main content
. 2008 Feb;172(2):299–308. doi: 10.2353/ajpath.2008.070057

Figure 8.

Figure 8

Snail mediates the TGF-β1-triggered P-cadherin and nephrin suppression in podocytes. A: RT-PCR shows that TGF-β1 induced Snail mRNA expression in a time-dependent manner. Podocytes were incubated with TGF-β1 (2 ng/ml) for various periods of time as indicated. B: Forced expression of Snail suppresses P-cadherin expression in podocytes. Podocytes were transiently transfected with Snail expression vector (pHA-Snail) for 72 hours. Snail, P-cadherin, and β-actin expression was assessed by RT-PCR. Podocytes transiently transfected with empty vector pcDNA3 plasmid were used as negative control, whereas podocytes treated with TGF-β1 served as a positive control. C and D: Ectopic expression of Snail inhibits nephrin expression in podocytes. C: Nephrin mRNA expression was assessed by RT-PCR. Quantification of nephrin mRNA abundance was performed after normalization with β-actin. Data are presented as mean ± SEM of three experiments. *P < 0.05 versus controls. †P < 0.05 versus pcDNA3 control.