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Comparison of the 1H NMR chemical shifts of wild-type α-sarcin and the L145F mutant. Chemical shift variations (Δδ = δmut − δwt) in the protein backbone resonances for Hα (α-protons) (A) and HN (peptide bond protons) (B), at pH 6.0 and 35°C, are shown along the protein sequence.
