Figure 2.

Histograms showing the stepwise appearance of the ¹⁵N-¹H HSQC cross-peaks of all-Ala α-lactalbumin (pH 2) as a function of pressure and temperature. The height of the bar indicates the pressure at which the cross-peak first appears. Cross-peaks are visible from residues 1, 2, 63–66, 68–76, 78 (16 peaks) at 30 bar, 20°C; from residues 1–3, 33, 36, 38, 41, 44–49, 61–66, 68–80 (32 peaks) at 1000 bar, 20°C; from residues 1–3, 33, 36–39, 41, 43–52, 57–66, 68–80 (42 peaks) at 1500 bar, 20°C; from residues 1–3, 33, 36–52, 57–66, 68–80 (44 peaks) at 2000 bar, 20°C; from residues 1–5, 7, 9–10, 13–14, 21, 33–66, 68–87, 102 (66 peaks) at 2000 bar, 36°C.