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Schematic illustration of the gradual unfolding of the molten globule of all-Ala α-lactalbumin (pH 2) with increasing pressure, drawn on the native structure of α-lactalbumin. Ribbons show residues whose signals are visible in the 15N-1H HSQC spectra. The colors indicate the pressure at which the signal begins to appear (red: 30 bar; green: 1000 bar; violet: 1500 bar; cyan: 2000 bar; blue: 2000 bar at −18°C or 36°C). Balls represent residues invisible in the 15N-1H HSQC-spectra.
