Table 1.
Residuea | ln(kA)b | Slopec | ln(kB)b |
Gly 90 | 2.26 | −7490 | 7.61 |
Gly 45 | 2.23 | −6778 | 7.06 |
Gly 37 | 2.00 | −3121 | 7.60 |
Ser 89 | 1.88 | −3391 | 7.51 |
Ile 87 | 1.61 | −3442 | 7.09 |
His 46 | 1.28 | −5996 | 7.11 |
Trp 48 | 0.74 | −6447 | – |
Thr 113 | 0.72 | −7959 | 6.19 |
Asn 47 | 0.58 | −6456 | 6.62 |
Cys 112 | 0.58 | −6690 | – |
His 117 | 0.57 | −7765 | – |
Phe 111 | 0.36 | −4173 | – |
Gly 9 | 0.29 | −1127 | – |
Asp 71 | 0.01 | −1127 | – |
Asn 42 | −0.32 | 2501 | – |
Thr 124 | −0.59 | 706 | – |
Gly 123 | −0.67 | 1488 | – |
Gly 58 | −0.82 | 3836 | – |
a Residues with well-defined kA values at all three temperatures are listed. Table rows are sorted with respect to decreasing values of ln(kA) at T = 35.4°C.
b The exchange rate constants kA and kB are forward and backward reaction parameters obtained by fitting to relaxation data as described in the text. Whenever the kB values are poorly defined, that is, they are smaller than their errors for at least one temperature, no value is given.
c Slope obtained for the values at 25.7 and 35.4°C (see text and Fig. 3 ▶).