Table 2.
Functional expression of A. thaliana AOX1a
| Expression plasmid | Uninduced | Induceda |
| pUNI-AtAO | ||
| Recombined with pHOST | ||
| pHB2-GST | +++ | o |
| pHB3-His6 | o | o |
| pHB-QE82 | o | ++ |
| pHB-GFPc3 | o | ++ |
| pBAD/thioE | +++ | ++++ |
| pHB-NUSc2 | +++ | +++inh |
| pHB-MALc2X | +++inh | oinh |
| pHB-MALc2M | o | ++++ |
| pHB-MALcM | o | ++++ |
| Nonrecombined | ||
| pAtAOmKX | ++++ | ++++ |
| pAtAOc2 | +++inh | oinh |
| pAtAOc2MED | + | +++++ |
| pAtAOcM | + | +++++ |
| No AOX gene | ||
| pUC118 | o | o |
| pBAD/thioE | o | o |
| pHB-MALc2X | o | o |
| pHB-MALcM | o | o |
The relative colony size of hemA−E. coli 64 h after plating is shown. “o” denotes background size of colony, which was visible only as a refractive difference at the surface of the agar. “+” through “+++++” denotes increasing size of visible colonies. An “inh” superscript indicates an inhomogeneity in colony size, with the median size reported.
a Induced with 0.1 mM IPTG or arabinose, as required by the promoter.