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Overexpression of A. thaliana AOX and PTOX
| Expressed level in: | |||
| Gene | pHOST | Membrane | Inclusion bodies |
| AOX | pHB2-GST | o | +++ |
| pHB3-His6 | o | ++ | |
| pHB-QE82 | o | o | |
| pBAD/thioE | o | +++ | |
| pHB-MALc2X | +++++ | ++++ | |
| pHB-MAL-T7 | o | o | |
| pHB-PET43 | o | o | |
| pHB-NUSc2 | ++ | ++ | |
| PTOX | pHB2-GST | + | +++ |
| pHB-QE82 | o | o | |
| pBAD/thioE | o | ++ | |
| pHB-MALc2X | ++++ | ++++ | |
Relative expression levels as observed on Coomassie-stained denaturing polyacrylamide gels. “o” denotes no obvious expression, and “+” through “++++,” increasing protein level.
