Table 1.

Design and target amino acid percentages for random sequence library

Amino acid	RNN (%)	NHY (%)	NNG (%)	Combined (%)	Target (%)	Difference (%)
Ala	12.5	8.25	6.3	9	8.5	0.5
Arg	6.3	0	12.5	6.3	4.6	1.7
Asn	6.3	8.3	0	4.8	4.7	0.1
Asp	6.3	8.3	0	4.8	6.0	−1.2
Cys	0	0	0	0	1.5	−1.5
Gln	0	0	12.5	4.2	6.0	−1.8
Glu	6.3	0	6.3	4.2	3.9	−0.3
Gly	12.5	0	6.3	6.3	7.8	−1.5
His	0	8.3	0	2.8	2.3	0.5
Ile	9.4	8.3	0	5.9	5.7	0.2
Leu	0	8.3	12.5	6.9	8.1	−1.2
Lys	6.3	0	6.3	4.2	5.7	−1.5
Met	3.1	0	6.3	3.2	2.1	1.1
Phe	0	8.3	0	2.8	4.0	−1.2
Pro	0	8.3	6.3	4.8	4.7	0.1
Ser	6.3	8.3	6.3	6.9	6.1	0.8
Thr	12.5	8.3	6.25	9.0	5.8	3.2
Trp	0	0	6.3	2.1	1.5	0.6
Tyr	0	8.3	0	2.8	3.9	−1.1
Val	12.5	8.3	6.3	9	6.9	2.1
Nonpolar (AILMFWV)	37.6	14.2	37.5	38.8	36.7	2.1
Acidic (DE)	12.5	8.3	6.3	9	9.9	−0.9
Basic (HKR)	12.5	8.3	18.8	13.2	12.6	0.6
Polar (DEHKNQR)	31.3	24.8	37.5	31.2	33.1	−1.9