Table 5.

Summary of sequence and biophysical characteristics of inserted sequences

Inserted sequence no.	Insert size (aa)	Insert recovery level (fold over backgrounda)	% Hydrophobic residues (inserted seq)	% Polar residues (inserted seq)	Expression level	Pellet/SN	ΔGunf (kcal/mole)	ΔΔGunf (kcal/mole)	SPR binding
none	0	280	—	—	high	10/90	1.5	—	++
217	60	9	24.1	36.1	high	80/20	0.8	−0.7	++
227	60	3	36.2	25.7	high	80/20	NMb	NM	−
283	80	5	19.2	34.5	high	80/20	1.7	+0.2	+
290	60	3700	17.2	32.6	high	50/50	0.9	−0.6	+
333	100	3	28.2	28.0	high	80/20	0.7	−0.8	+
344	60	9	18.9	32.6	high	50/50	—	—	NM
425	60	770	24.1	27.4	high	50/50	1.3	−0.2	+

^a Background recovery was taken to be recovery levels of phage displaying the protein L domain.

^b Not measured.