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The general catalytic mechanism for retaining glycosyl hydrolases. (I) Protonation of the glycosidic oxygen by the proton donor (Glu 35) and attack on the glucose C1 by the nucleophile (Asp 52). Departure of the reducing end of the substrate. (II) Activation of a water molecule, cleavage of C1-Asp 52 covalent bond. (III) Regeneration of the initial protonation states.
