Figure 1.

Residues 11–51 of the Par-4 WT peptide in a helical wheel format. Positions in the helical wheel are denoted by abcdefg. The Leucine repeat at the d position is shown in bold black letters. Acidic residues are red, basic residues blue. The acidic repeat at the g position is in bold red italics. The black dashed arrows mark selected e–g′ and e′–g interhelical contacts. The gray dashed arrows mark several possible intrahelical charge repulsions involving e and g residues. The residues targeted for mutagenesis are boxed. The substitutions used to create the four mutants in this study are specified at the bottom of the figure.