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. 2003 Mar;12(3):551–559. doi: 10.1110/ps.0233003

Figure 1.

Figure 1.

Reducing SDS-PAGE analysis of expression levels of MHC-I heavy chains in Escherichia coli. (A) Expression levels of rA2. (B) Expression levels of rKk (des cys). (C) Expression levels of rA11. Fermentor samples (15 μL) were withdrawn before and every hour after induction. The bacterial cell pellet was resuspended in 50 μL MgCl2/SDS lysis buffer to release and solubilize heavy-chain inclusion bodies as described by Chen and Christen (1997). After centrifugation at 20,000g for 2 min, 15 μL of the supernatant was loaded directly on the gel. Lane 1, protein marker; lane 2, before induction; and lanes 3–5, samples taken 1, 2, and 3 h after induction with IPTG, respectively. Positions of heavy-chain monomers are shown with arrows.