Figure 2.

Reducing and non-reducing SDS-PAGE analysis of solubilized inclusion body preparations. (A) Solubilized inclusion bodies containing rA2. (B) Solubilized inclusion bodies containing rA11. The intensity of the heavy-chain monomer band increased on reduction, owing to the release of disulfide bond cross-linked monomers, and made the distinction of the isomers difficult. To improve the visualization of heavy-chain isomers, reducing samples of rA2 and rA11 were diluted twice as much in sample buffer as the non-reducing counterparts. The positions of heavy-chain isomers 0, 1, and 2 are indicated on the figure. NR indicates non-reducing; R indicates reducing.