Figure 3.

Separation of rA2 heavy-chain isomers by hydrophobic interaction chromatography on phenyl Sepharose High Performance under non-reducing denaturing conditions. Aliquots (7.5 μL) of fractions collected in the shaded area of the chromatogram were analyzed by non-reducing SDS-PAGE, and the result is shown below. Analyzed fractions are indicated on the figure, as well as the positions of heavy-chain isomers. Lane M, protein marker; lane L, sample applied on the hydrophobic interaction chromatography column.