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Summary of the differences in binding free energy measurements (ΔΔ Gbinding) between Ala/Gly SosY peptide mutants bound with different Sem-5 C-SH3 domain mutants
| Sem-5 C-SH3 | ΔΔGbinding, SosY-P3Ala → Gly (cal/mole) | ΔΔGbinding, SosY-P6Ala → Gly (cal/mole) |
| S170A | −565 | −533 |
| S170G | −642 | −534 |
| G171 | −658 | −528 |
| Average | −622 ± 48 | −532 ± 43 |
