Figure 2.

Experimental and calculated ¹H/¹⁵N PISEMA spectra for the TM region of the fd coat protein, in aligned lipid bilayers selectively ¹⁵N-labeled with (A) Ala, (B) Gly, (C) Leu, and (D) Val. The resonances were assigned to specific amino acids in the sequence by comparison of the experimental spectrum (top) with the Pisa wheel spectrum (bottom). In each panel, the Pisa wheel PISEMA spectrum was calculated from a protein model with the IP helix tilted by τ = 87°, and the TM tilted by τ = 30°.