Inhibition of [3H]PGE1 binding to control platelets by IgG protein identified in SCI plasma. Control, non-SCI, washed platelets (2 × 108 cells per ml) in Tyrode’s buffer, pH 7.4, containing 5 mM MgCl2 were incubated for 3 hr at 37°C with increasing concentrations of the IgG protein. After incubation, the specific binding of [3H]PGE1 was determined (5). X, eluates from control plasma; •, eluates from SCI plasma.