Figure 2.

PCR detection and analysis of AAV–AAVS1 junctions. (a) Substrates tested in the integration assay are shown. (b) The integration assay was carried out with the HT-AAV substrate in the presence (+) or absence (−) of Rep. The integration products were detected by PCR using the primers BAAV4 and H5d and analyzed by gel electrophoresis. (c) Southern blot hybridization with an AAV probe (AAV D). (d) Southern blot hybridization with an AAVS1 probe (H4d1). Lane M contains the digoxigenin-labeled DNA molecular weight marker VI from Boehringer Mannheim.