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Structures of the AAV–AAVS1 junctions generated in the integration assay. (a) The regions of both substrates involved in formation of the AAV–AAVS1 junctions are shown. The elements TRS and RBS are indicated with their respective nucleotide positions. (b) AAV–AAVS1 junctions with the crossover point within the RBS of AAV and AAVS1. (c) AAV–AAVS1 junctions with the crossover point outside of the RBS of AAV and AAVS1 or outside of AAVS1 only.
