Figure 4.

Clustering of GABA_A receptors mediated by the endogenous γ3 subunit. The γ3 subunit in the RTN promotes gephyrin and GABA_A receptor α3 subunit clustering in γ2^0/0 mice. Parasagittal sections through the RTN of P14 mice (n = 4–6 per genotype) were double stained for the α3 and γ3 subunit (a and c; α3 red, γ3 green) or the α3 subunit and gephyrin (b and d; α3 red, gephyrin green) and visualized by confocal microscopy. Yellow puncta in the superimposed red and green images illustrate colocalization of the markers used. In γ2^0/0 RTN, the α3 subunit IR was colocalized with strong IR for the γ3 subunit (a), as well as for gephyrin (b), whereas in γ2^+/+ RTN, there was only a partial colocalization of the α3 subunit IR and the weaker γ3 subunit IR (c), but a prominent colocalization of the gephyrin and α3 subunit IR (d). (Scale bar, 10 μm.)