Figure 3.

Southern blot analysis of genomic DNA from: C57BL/6J +/+ (B6); C57BL/6J ep/ep congenic (ep); and C3H/HeJ +/+ (C3H) digested with BglII and EcoRI. The blots were hybridized with a 1.2-kb ScaI fragment derived from the MEP-2 cDNA. Selected size markers at right indicate the position of lambda DNA fragments digested with BstEII. Note the presence of exon-bearing fragments of altered size in ep DNA (BglII: ≈4.0 kb; EcoRI: ≈9.0 kb) shown in lanes 2 and 5, respectively. The variation seen in the sizes of EcoRI-digested DNA between wild-type C57BL/6J and C3H/HeJ represents a restriction fragment length polymorphism. The position of marker fragments (in kilobases) is shown on the right.