Figure 5.

Expression of the ep gene. (A) Northern blot of poly(A)⁺ RNAs (6 μg) isolated from brain or liver of homozygous wild-type (wt) or homozygous ep (ep) and hybridized with a 0.75-kb SmaI fragment of cDNA MEP-1 (Upper), or control probe from Gapd cDNA (Lower). Note the apparent size increase in all forms of the ep transcript compared with those observed in wild-type brain and liver RNA. (B) Mouse multiple tissue Northern blot (CLONTECH). Lanes: 1, 2 μg of poly(A)⁺ RNA from wild-type heart; 2, brain; 3, spleen; 4, lung; 5, liver; 6, skeletal muscle; 7, kidney; and 8, testes hybridized with a 0.75-kb SmaI fragment of cDNA MEP-1. Control hybridization to β-actin (not shown) confirmed the integrity and equal loading of all RNAs. For A and B, the sizes of marker RNAs are shown to the right in kilobases.