Figure 2.

Identification of a cDNA band displaying a paternal expression pattern. Typical AMD patterns of brain RNAs from four different mice, JF, B6, (JF × B6) F₁, and (B6 × JF) F₁ (from left to right in each quartet), are shown. Because different primer combinations are used in odd- and even-numbered quartets, two unique fingerprints were alternately displayed. The band indicated by the arrow in each odd-numbered quartet is a B6-specific allele termed MIG26 displaying paternal expression pattern that was detected using GT₁₅G and GGNYGCGATC. Note that each quartet represents the pattern from an independent set of animals, thereby confirming the reproducibility of the expression pattern of MIG26. [The very faint band observed in some (B6 × JF) F₁ animals may be a fragment comigrating with, but distinct from, MIG26, since the results shown in Fig. 5 indicated that the possibility of leaky imprinting is highly unlikely.]