Figure 2.

Analysis of adenoviral transgene expression using βGal immunohistochemistry. Pictures of 14-μm-thick coronal sections through the caudate putamen (A) and SN (B) showing βGal-expressing cells 4 weeks after intrastriatal injection of Ad-βGal. Anti-E. coli βGal antibodies were used to distinguish the transgenic from the endogenous βGal activity. In the striatum, βGal⁺ cells are found along the needle tract (indicated by arrows in A) and up to 2 mm from the site of injection. Numerous infected cells can be observed in the SN compacta (B) after retrograde transport of viral particles delivered in the caudate putamen. (Bar = 200 μm.)