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P21/waf1 is recruited to HIV-1 preintegration complex (PIC). ACH2 cells were treated with 9AA or DMSO as mock control. Cells were then harvested, lysed and submitted to immunoprecipitation with anti-MA or anti-RT (ABI Inc., Columbia, MD). The immunopreciptated complexes were separated on 4–20% SDS-PAGE gels and then submitted to western blot to detect the presence p21/waf1. P21/waf1 is found to be present in the anti-MA immunoprecipitation complex.
